Experimental Biochemistry Online Course | IIT Kharagpur NPTEL | Prof. Swagata Dasgupta
Course Details
| Exam Registration | 177 |
|---|---|
| Course Status | Ongoing |
| Course Type | Core |
| Language | English |
| Duration | 12 weeks |
| Categories | Chemistry, Biosciences |
| Credit Points | 3 |
| Level | Undergraduate |
| Start Date | 19 Jan 2026 |
| End Date | 10 Apr 2026 |
| Enrollment Ends | 02 Feb 2026 |
| Exam Registration Ends | 20 Feb 2026 |
| Exam Date | 25 Apr 2026 IST |
| NCrF Level | 4.5 — 8.0 |
Unlock the Secrets of the Lab: A Premier Course in Experimental Biochemistry
For any aspiring biochemist, molecular biologist, or chemist, theoretical knowledge is only half the battle. True mastery comes from hands-on experience in the laboratory. Recognizing this critical need, the Indian Institute of Technology Kharagpur (IIT Kharagpur) offers an exceptional online course: Experimental Biochemistry. This meticulously designed 12-week program, available through the National Program on Technology Enhanced Learning (NPTEL), provides undergraduate students with a comprehensive foundation in essential biochemistry laboratory techniques.
Learn from Distinguished IIT Kharagpur Faculty
This course is led by two eminent professors whose combined expertise spans decades of teaching and groundbreaking research.
Prof. Swagata Dasgupta is a renowned figure in protein chemistry at IIT Kharagpur. With a Ph.D. from Rensselaer Polytechnic Institute, USA, and over 25 years of service, her research focuses on protein-protein and protein-small molecule interactions. An award-winning scientist and Fellow of the Indian Academy of Sciences, she has authored over 150 publications. Prof. Dasgupta is also a dedicated educator, having developed popular video courses that have reached a global audience.
Prof. Soumya De, an Associate Professor in Bioscience and Biotechnology, brings a dynamic perspective from the interface of biology and physics. After his Ph.D. from Cornell University and post-doctoral work in Canada, his lab now focuses on structural biology, protein engineering, and cancer research. His experience in teaching introductory biology ensures the course content is accessible and engaging for first-year students.
Course Overview: What You Will Learn
This undergraduate-level course is structured to take you from fundamental concepts to advanced applications, preparing you for future research and development roles in biotechnology and pharmaceutical industries.
Intended Audience: Students of Chemistry, Biochemistry, Biotechnology, and related biological sciences.
Prerequisites: A basic understanding of Biochemistry is desirable.
Duration: 12 Weeks
Detailed 12-Week Course Layout
| Week | Topic | Key Learning Outcomes |
|---|---|---|
| 1 | Basics of Experimental Biochemistry | Introduction to lab safety, instrumentation, and good laboratory practices. |
| 2 | Amino Acid Titration and pI Determination | Understand acid-base properties of amino acids and isoelectric point. |
| 3 | Spectroscopic Techniques | Hands-on with UV-Vis, fluorescence spectroscopy for biomolecule analysis. |
| 4 | Protein Folding and Denaturation Studies | Explore factors affecting protein structure and stability. |
| 5 | Chromatographic Techniques | Learn separation methods like Gel Filtration, Ion-Exchange, and Affinity Chromatography. |
| 6 | Gel Electrophoresis of DNA and Proteins | Master SDS-PAGE, Native PAGE, and Agarose Gel Electrophoresis. |
| 7 | Isolation and Characterization of Proteins | Practical protocols for protein purification and analysis. |
| 8 | Enzyme Kinetics | Determine Michaelis-Menten parameters and study enzyme inhibition. |
| 9 | Isolation and Characterization of DNA | Techniques for DNA extraction, quantification, and quality assessment. |
| 10 | Basics of rDNA Technology | Introduction to recombinant DNA techniques and applications. |
| 11 | Protein-Ligand Interactions | Study binding phenomena using various biophysical methods. |
| 12 | Immunoassay Techniques | Learn ELISA and other antibody-based detection methods. |
Why Enroll in This Course?
- Industry-Relevant Skills: Directly applicable in Biotech & Pharma companies.
- Expert Demonstration: Each topic includes theoretical overviews followed by actual experiment demonstrations.
- Structured Learning: Weekly assignments help reinforce concepts and track progress.
- Foundation for Research: Perfect preparation for higher studies and R&D projects.
- Global Access: As an NPTEL course, it's freely accessible to students and educators worldwide.
Recommended Textbook
The course aligns with the concepts in Modern Experimental Biochemistry by Rodney F. Boyer, a classic text that serves as an excellent companion for deeper understanding.
Whether you aim to strengthen your lab skills for academic success or build a career in the life sciences industry, Experimental Biochemistry from IIT Kharagpur is an invaluable resource. Enroll today and take the first step towards becoming a proficient experimental biochemist.
Enroll Now →